Antioxidant Activity of the Prunus mahaleb Seed Oil Extracts Using n-Hexane and Petroleum Ether Solvents: In Silico and In Vitro Studies

Prunus mahaleb L., also known as white mahaleb, and native to the Kurdistan region of Iraq, has significant nutraceutical therapeutic ingredients. The seeds are rich in conjugated fatty acids with small quantities cyanogenic glycosides, coumarin derivatives, flavonoids. contents were extracted Soxhlet apparatus using n-hexane petroleum ether solvents, separately. Gas chromatography-mass spectrometry (GC-MS) was used recognize chemical composition compounds. radical scavenging activity performed for total extracts from solvents 2,2-diphenyl-1 picrylhydrazyl (DPPH) assay compared quercetin a positive control. Furthermore, molecular docking identified compounds against five enzymes that have main roles intracellular oxidation. Afterwards, drug-like properties bioactivity predictions applied all Molinspiration software. results showed four phthalate six saturated (SFAs), monounsaturated (MUFAs), three polyunsaturated (PUFAs). extract competitive antioxidant in-silico studies suggested notable seed oil apparent drug-likeness properties. Further required separate extracts, then perform vitro on